Anti-MAT1A Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q00266
Gene Id	4143
Gene Symbols	MAT1A
Protein Name / Synonyms	MAT1A, MAT, MATA1, SAMS, SAMS1, methionine adenosyltransferase 1A
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene catalyzes a two-step reaction that involves the transfer of the adenosyl moiety of ATP to methionine to form S-adenosylmethionine and tripolyphosphate, which is subsequently cleaved to PPi and Pi. S-adenosylmethionine is the source of methyl groups for most biological methylations. The encoded protein is found as a homotetramer (MAT I) or a homodimer (MAT III) whereas a third form, MAT II (gamma), is encoded by the MAT2A gene. Mutations in this gene are associated with methionine adenosyltransferase deficiency.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

43kDa

Observed MW

44kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-395 of human MAT1A (NP_000420.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using MAT1A antibody (144-61348) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
Western blot - MAT1A antibody (144-61348)

Immunohistochemistry of paraffin-embedded human liver injury using MAT1A antibody (144-61348) at dilution of 1:100 (40x lens).
Immunohistochemistry - MAT1A antibody (144-61348)

Expiration:

12 months from the date of shipment when stored properly.

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