3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-MB Antibody
Catalog #: 130-10068
Rabbit Anti-Human MB Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P02144 |
| Gene Id | 4151 |
| Gene Symbols | MB |
| Clonality | Polyclonal |
| Immunogen | Immunogen was recombinant protein derived from human MB. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1: 80, 000), Western Blotting (recommended work dilution= 1:1000) , Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein MB, Lane 2, mouse heart cell lysate, Lane 3, mouse muscle cell lysate. The membrane blot was probed with antiMB primary antibody (1 µg/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate cellular from Mouse cells (17 kDa). |
| Antigen Catalog Number | 230-00032 |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Research Area | Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Myoglobin (MB) is an iron- and oxygen-binding protein found in the muscle tissue of vertebrates and almost all mammals. Myoglobin is a single-chain globular protein containing a heme (iron containing porphyrin) prosthetic group in the center. The oxygen binding of myoglobin is unaffected by the oxygenpressure in the surrounding tissue. The only releasing time of myoglobin in the bloodstream happens after muscle injury. If myoglobin presents in blood, it will be considered to be abnormal.
Specificity
This antibody binds to Human Myoglobin. Cross reactivity with rat are expected from sequence similarity.
Storage
Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilizedantibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can be aliquoted and stored frozen at -20°C to -80°C in a manual defrost freezer for at least 6 months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for 1 month at 4°C. Please avoid freeze-thaw cycles, as this will lower the activity of the antibody.
Related Products
Recombinant Human Myoglobin (catalog #: 230-00032) Mouse Anti-Myoglobin Cardiac (catalog #: MD-17-0070)
References
- Flonta SE, et al. (2009) Expression and functional regulation of myoglobin in epithelial cancers. Am J Pathol. 175(1):201-6.
- Weber MA, et al. (2007) Myoglobin plasma level related to muscle mass and fiber composition: a clinical marker of muscle wasting? J Mol Med (Berl). 85(8):887-96.
Expiration:
12 months from the date of shipment when stored properly.