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Anti-MB Antibody, Biotinylated
Catalog #: 130-10068B-50
Rabbit anti-MB Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P02144 |
| Gene Id | 4151 |
| Gene Symbols | MB |
| Clonality | Polyclonal |
| Immunogen | Immunogen was recombinant protein derived from human MB. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1: 80, 000), Western Blotting (recommended work dilution= 1:1000) , Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein MB, Lane 2, mouse heart cell lysate, Lane 3, mouse muscle cell lysate. The membrane blot was probed with antiMB primary antibody (1 µg/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate cellular from Mouse cells (17 kDa). |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Myoglobin (MB) is an iron- and oxygen-binding protein found in the muscle tissue of vertebrates and almost all mammals. Myoglobin is a single-chain globular protein containing a heme (iron containing porphyrin) prosthetic group in the center. The oxygen binding of myoglobin is unaffected by the oxygenpressure in the surrounding tissue. The only releasing time of myoglobin in the bloodstream happens after muscle injury. If myoglobin presents in blood, it will be considered to be abnormal.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
This antibody binds to Human Myoglobin. Cross reactivity with rat are expected from sequence similarity.
Related Products
Recombinant Human Myoglobin (catalog #: 230-00032) Mouse Anti-Myoglobin Cardiac (catalog #: MD-17-0070)
References
- Flonta SE, et al. (2009) Expression and functional regulation of myoglobin in epithelial cancers. Am J Pathol. 175(1):201-6.
- Weber MA, et al. (2007) Myoglobin plasma level related to muscle mass and fiber composition: a clinical marker of muscle wasting? J Mol Med (Berl). 85(8):887-96.
Expiration:
12 months from the date of shipment when stored properly.