Anti-MBD2 Antibody

Product Description

Specifications

Size	200 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Mouse
Accession Number	Q9UBB5
Gene Id	8932
Gene Symbols	MBD2
Protein Name / Synonyms	Methyl-CpG-binding domain protein 2, Demethylase, DMTase, Methyl-CpG-binding protein MBD2, MBD2
Clonality	Monoclonal
Clone	1812CT625.9.45
Isotype	Kappa, IgG1
Recommended Applications	IHC-P, Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.

Other Information

Antigen Type

Recombinant Protein

Format

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

Calculated Molecular Weight (Da)

43255

Antigen Source

Human

Images

IHC-P (1:25)

102-20291 staining MBD2 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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