RayBiotech

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Anti-MCCC2 Antibody

Catalog #: 144-07990

MCCC2 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
Q9HCC0
Gene Id
64087
Gene Symbols
MCCC2
Protein Name / Synonyms
MCCC2, MCCB, methylcrotonoyl-CoA carboxylase 2
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes the small subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW
57kDa/61kDa
Observed MW
61kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 284-563 of human MCCC2 (NP_071415.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using MCCC2 antibody (144-07990) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - MCCC2 Polyclonal Antibody (144-07990)

Western blot analysis of extracts of various cells, using MCCC2 antibody (144-07990) at 1:1000 dilution ratio through one-step method.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:4000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - MCCC2 Polyclonal Antibody (144-07990)

Immunohistochemistry of paraffin-embedded human prostate using MCCC2 antibody (144-07990) at dilution of 1:100 (40x lens).
Immunohistochemistry - MCCC2 Polyclonal Antibody (144-07990)

Immunohistochemistry of paraffin-embedded rat brain using MCCC2 antibody (144-07990) at dilution of 1:100 (40x lens).
Immunohistochemistry - MCCC2 Polyclonal Antibody (144-07990)

Expiration:
12 months from the date of shipment when stored properly.