MCD antibody [N2C1], Internal, 1.01 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 23417 |
| Protein Name / Synonyms | MCD, Internal |
| Concentration (lot specific) | 1.01 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
MCD antibody, MGC59795 antibody, MLYCD antibody, malonyl coenzyme A decarboxylase antibody, "malonyl-CoA decarboxylase, mitochondrial antibody", malonyl-CoA decarboxylase antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
55 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 90 and 304 of (Uniprot ID# O95822)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
MCD antibody [N2C1], Internal detects MCD protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
MCD antibody [N2C1], Internal (107-10891) dilution: 1:500.
Sample: Paraffin-embedded mouse kidney.
MCD antibody [N2C1], Internal (107-10891) dilution: 1:500.
MCD antibody [N2C1], Internal detects MCD protein at cytosol on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
MCD antibody [N2C1], Internal (107-10891) dilution: 1:500.
Sample: Paraffin-embedded mouse muscle.
MCD antibody [N2C1], Internal (107-10891) dilution: 1:500.
MCD antibody detects MCD protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract
D. 30 µg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
MCD antibody (107-10891) dilution: 1:500
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract
D. 30 µg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
MCD antibody (107-10891) dilution: 1:500
MCD antibody detects MCD protein by western blot analysis.
A. 30 µg C8D30 whole cell lysate/extract
7.5 % SDS-PAGE
MCD antibody (107-10891) dilution: 1:500
A. 30 µg C8D30 whole cell lysate/extract
7.5 % SDS-PAGE
MCD antibody (107-10891) dilution: 1:500
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MCD(107-10891) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded H661 xenograft, using MCD(107-10891) antibody at 1:500 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.