Anti-MCM3 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	4172
Protein Name / Synonyms	MCM3
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

HCC5 antibody, MGC1157 antibody, P1-MCM3 antibody, P1.h antibody, RLFB antibody, MCM3 antibody, RLF subunit beta antibody, MCM3 minichromosome maintenance deficient 3 antibody, minichromosome maintenance deficient 3 antibody, DNA replication factor MCM3 antibody, DNA polymerase alpha holoenzyme-associated protein P1 antibody, p102 antibody, hRlf beta subunit antibody, "replication licensing factor, beta subunit antibody", cervical cancer proto-oncogene 5 antibody, DNA replication licensing factor MCM3 antibody, minichromosome maintenance complex component 3 antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

91 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 42 and 302 of MCM3 (Uniprot ID# P25205)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:5000-1:20000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MCM3(107-10953) antibody at 1:500 dilution.

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using MCM3(107-10953) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: Hep G2
7.5% SDS PAGE
107-10953 diluted at 1:10000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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