MCM7 antibody [N2C2], Internal, 0.76 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 4176 |
| Protein Name / Synonyms | MCM7, Internal |
| Concentration (lot specific) | 0.76 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
CDC47 antibody, MCM2 antibody, P1.1-MCM3 antibody, P1CDC47 antibody, P85MCM antibody, PNAS146 antibody, MCM7 antibody, homolog of S. cerevisiae Cdc47 antibody, minichromosome maintenance deficient 7 antibody, CDC47 homolog antibody, DNA replication licensing factor MCM7 antibody, minichromosome maintenance complex component 7 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
81 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 190 and 481 of MCM7 (Uniprot ID# P33993)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Cross-linked ChIP was performed with HeLa chromatin extract and 5 µg of either control rabbit IgG or anti-MCM7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.
MCM7 antibody [N2C2], Internal immunoprecipitates MCM7 protein in IP experiments.
IP samples: HeLa whole cell extract
A. 40 µg HeLa whole cell extract
B. Control with 4 µg of preimmune Rabbit IgG
C. Immunoprecipitation of MCM7 protein by 4 µg MCM7 antibody [N2C2], Internal (107-10910)
7.5 % SDS-PAGE
The immunoprecipitated MCM7 protein was detected by MCM7 antibody [N2C2], Internal (107-10910) diluted at 1:1000.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]
IP samples: HeLa whole cell extract
A. 40 µg HeLa whole cell extract
B. Control with 4 µg of preimmune Rabbit IgG
C. Immunoprecipitation of MCM7 protein by 4 µg MCM7 antibody [N2C2], Internal (107-10910)
7.5 % SDS-PAGE
The immunoprecipitated MCM7 protein was detected by MCM7 antibody [N2C2], Internal (107-10910) diluted at 1:1000.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MCM7(107-10910) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using MCM7(107-10910) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
107-10910 diluted at 1:1000
A: H1299
7.5% SDS PAGE
107-10910 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.