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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 4191 |
| Protein Name / Synonyms | MDH2 |
| Concentration (lot specific) | 0.76 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
M-MDH antibody, MDH antibody, MGC:3559 antibody, MOR1 antibody, MDH2 antibody, malate dehydrogenase, mitochondrial antibody, malate dehydrogenase 2, NAD (mitochondrial) antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
36 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 40 and 319 of MDH2 (Uniprot ID# P40926)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
MDH2 antibody detects MDH2 protein at cytosol on H1299 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H1299 xenograft.
MDH2 antibody (107-10565) dilution: 1:500.
Sample: Paraffin-embedded H1299 xenograft.
MDH2 antibody (107-10565) dilution: 1:500.
MDH2 antibody detects MDH2 protein at mitochondrion on mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
MDH2 antibody (107-10565) dilution: 1:500.
Sample: Paraffin-embedded mouse pancreas.
MDH2 antibody (107-10565) dilution: 1:500.
MDH2 antibody detects MDH2 protein at mitochondrion on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
MDH2 antibody (107-10565) dilution: 1:500.
Sample: Paraffin-embedded mouse kidney.
MDH2 antibody (107-10565) dilution: 1:500.
Sample (20 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
107-10565 diluted at 1:10000
A: mouse brain
10% SDS PAGE
107-10565 diluted at 1:10000
MDH2 antibody detects MDH2 protein by Western blot analysis.
A. 50 µg rat lung lysate/extract
10 % SDS-PAGE
MDH2 antibody (107-10565) dilution: 1:5000
A. 50 µg rat lung lysate/extract
10 % SDS-PAGE
MDH2 antibody (107-10565) dilution: 1:5000
Sample(30 µg of whole cell lysate)
A:293T
12% SDS PAGE
107-10565 diluted at 1:1000
A:293T
12% SDS PAGE
107-10565 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.