Anti-Mdm2 (C-term) Antibody

Product Description

Specifications

Size	200 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q00987
Gene Id	4193
Gene Symbols	MDM2
Protein Name / Synonyms	Mdm2 (C-term)
Clonality	Polyclonal
Preparation	This Mdm2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 393-424 amino acids from the C-terminal region of human Mdm2.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Cardiovascular Disease, Cancer, p53 Signaling, DNA Damage
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

MDM2 is a target of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of MDM2 can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle,apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5.

Specificity

Other Names

MDM2; E3 ubiquitin-protein ligase Mdm2; Double minute 2 protein; Oncoprotein Mdm2; p53-binding protein Mdm2

NCBI Accession #

NP_001138811.1;NP_001265391.1;NP_002383.2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

55233

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	Western blot analysis of MDM2 (arrow) using rabbit polyclonal Mdm2 Antibody (C-term) (Cat# 102-11624). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MDM2 gene (Lane 2) (Origene Technologies).
	Western blot analysis of anti-Mdm2 Pab (Cat# 102-11624) in mouse lung tissue lysate. Mdm2 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
	MDM2 Antibody (Q213) (Cat# 102-11624) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the MDM2 antibody detected the MDM2 protein (arrow).
	Mdm2 Antibody (C-term) (Cat# 102-11624) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Mdm2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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