Anti-MDM2 (S166) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q00987
Gene Id	4193
Gene Symbols	MDM2
Protein Name / Synonyms	MDM2 (S166)
Clonality	Polyclonal
Preparation	This MDM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 141-176 amino acids from human MDM2.
Concentration (lot specific)	0.480 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Research Area	Cardiovascular Disease, Cancer, p53 Signaling, DNA Damage
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

MDM2 is a target of the transcription factor tumor protein p53. This protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of MDM2 can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle,apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5.

Specificity

Other Names

MDM2; E3 ubiquitin-protein ligase Mdm2; Double minute 2 protein; Oncoprotein Mdm2; p53-binding protein Mdm2

NCBI Accession #

NP_001138811.1;NP_001265391.1;NP_002383.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

55233

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50

	Western blot analysis of MDM2 antibody (S166) (Cat# 102-11620) in 293 cell line lysates (35ug/lane). MDM2 (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with the MDM2 Antibody (S166) (Cat# 102-11620), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of MDM2 Antibody (S166) (Cat# 102-11620) with Hela cell followed by Alexa Fluor^® 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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