Anti-Me2-H4 (K20) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P62805
Gene Id	8370
Gene Symbols	HIST1H4A
Protein Name / Synonyms	Me2-H4(K20)
Clonality	Polyclonal
Preparation	This H4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from human H4.
Concentration (lot specific)	0.264 mg/ml
Recommended Applications	Dot Blotting, IHC-P, FC, Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones.

Specificity

Other Names

HIST1H4A; H4/A; H4FA; Histone H4

NCBI Accession #

NP_001029249.1;NP_003486.1;NP_003529.1;NP_003530.1;NP_003531.1;NP_003532.1;NP_003533.1;NP_003534.1;NP_003535.1;NP_003536.1;NP_003537.1;NP_003539.1;NP_068803.1;NP_778224.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

11367

Recommended Dilutions

WB: 1:1000; DB: 1:500; IHC: 1:50-100; FC: 1:10-50

	Western blot analysis of lysate from K562 cell line, using H4 Antibody (K20)(Cat# 102-15514). 102-15514 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
	Dot blot analysis of anti-hH4-K20(Methyl 2) methylation-specific Pab (Cat. 102-15514) on nitrocellulose membrane. 50ng of methylation-peptide or Non methylation-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
	Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PSMA4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	H4 Antibody ( K20 [Me2] ) (Cat# 102-15514) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products