Anti-MEK1 (MAP2K1) (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q02750
Gene Id	5604
Gene Symbols	MAP2K1
Protein Name / Synonyms	MEK1 (MAP2K1) (N-term)
Clonality	Polyclonal
Preparation	This MEK1 (MAP2K1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MEK1 (MAP2K1).
Concentration (lot specific)	0.45 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Cardiovascular Disease, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

MAP2K1 is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.

Specificity

Predicted Reactivity

Rat, Rabbit

Other Names

MAP2K1; MEK1; PRKMK1; Dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAPK/ERK kinase 1

NCBI Accession #

NP_002746.1

Other Accession #

Q01986; P29678; P31938

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

43439

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of lysates from Jurkat,rat PC-12 and C6 cell line,mouse brain tissue (from left to right),using MAP2K1 Antibody (P1)(Cat# 102-17725).102-17725 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
	Western blot analysis of lysates from Jurkat,rat PC-12,mouse NIH/3T3,rat C6 cell line and mouse brain tissue (from left to right),using MAP2K1 Antibody (P1)(Cat# 102-17725).102-17725 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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