Anti-MEK2 (MAP2K2) (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P36507
Gene Id	5605
Gene Symbols	MAP2K2
Protein Name / Synonyms	MEK2 (MAP2K2) (Center)
Clonality	Polyclonal
Preparation	This MEK2 (MAP2K2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 262-292 amino acids from the Central region of human MEK2 (MAP2K2).
Concentration (lot specific)	0.500 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

MAP2K2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax.

Specificity

Other Names

MAP2K2; MEK2; MKK2; PRKMK2; Dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 2; MAPK/ERK kinase 2

NCBI Accession #

NP_109587.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

44424

Recommended Dilutions

IF: 1:10-50; WB: 1:1000; IHC: 1:50-100

	Fluorescent image of U251 cell stained with MEK2 (MAP2K2) Antibody (Center)(Cat# 102-17729/SA110819AW).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MEK2 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). MEK2 immunoreactivity is localized to Cytoplasm significantly.
	The anti-MAP2K2 Pab (Cat# 102-17729) is used in Western blot to detect MAP2K2 in mouse liver tissue lysate.
	Western blot analysis of MAP2K2 (arrow) using MAP2K2 Antibody (Center) (Cat# 102-17729).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K2 gene (Lane 2) (Origene Technologies).
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	MAP2K2 Antibody (A278) (Cat# 102-17729) western blot analysis in U937 cell line lysates (35ug/lane).This demonstrates the MAP2K2 antibody detected the MAP2K2 protein (arrow).