Anti-MEK2 (MAP2K2) (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P36507
Gene Id	5605
Gene Symbols	MAP2K2
Protein Name / Synonyms	MEK2 (MAP2K2) (N-term)
Clonality	Polyclonal
Preparation	This MEK2 (MAP2K2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MEK2 (MAP2K2).
Concentration (lot specific)	0.500 mg/ml
Recommended Applications	Western Blotting, IF
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

MAP2K2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax.

Specificity

Other Names

MAP2K2; MEK2; MKK2; PRKMK2; Dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 2; MAPK/ERK kinase 2

NCBI Accession #

NP_109587.1

Other Accession #

P36506; Q63932

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

44424

Recommended Dilutions

WB: 1:1000; IF: 1:10-50

MEK2 (MAP2K2) Antibody (N-term) (Cat# 102-17728) western blot analysis in Hela,MCF-7,rat C6 cell line and mouse Liver,rat Brain tissue lysates (35ug/lane).This demonstrates the MEK2 (MAP2K2) antibody detected the MEK2 (MAP2K2) protein (arrow).

Fluorescent confocal image of U251 cell stained with MEK2 (MAP2K2) Antibody (N-term)(Cat# 102-17728).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MEK2 (MAP2K2) primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).MEK2 (MAP2K2) immunoreactivity is localized to Cytoplasm significantly.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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