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Anti-mEOS2 Antibody, Biotinylated
Catalog #: 130-00026B-50
Mouse anti-mEOS2 Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Coral |
| Host Species | Mouse |
| Clonality | Monoclonal |
| Immunogen | The immunogen was recombinant Meso2 GFP. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein A / G affinity chromatography. |
| Isotype | IgG2a |
| Recommended Applications | ELISA (recommended work dilution= 1: 64, 000) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Monomeric (m)Eos2 is an engineered photoactivatable fluorescent protein widely used for superresolution microscopy. An optical highlighter isolated from another stony coral (Lobophyllia hemprichii), the tetrameric EosFP (named after the goddess of dawn in Greek mythology), behaves similarly to Kaede and the other variants described above. EosFP emits bright green fluorescence at 516 nanometers, and can be photoconverted to orange-red (581 nanometers) fluorescence when illuminated at near-ultraviolet wavelengths. Random and site-directed mutagenesis of tetrameric EosFP was used to generate two dimers and a true monomeric protein named mEosFP.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
This antibody binds specifically to Meso2 GFP.
References
- Mingshu, Zhang; et al. (2012) Rational design of true monomeric and bright photoactivatable fluorescent proteins. Nature Methods, doi:10.1038/nmeth.2021
Expiration:
12 months from the date of shipment when stored properly.