Anti-MERTK Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q12866
Gene Id	10461
Gene Symbols	MERTK
Protein Name / Synonyms	MERTK
Clonality	Polyclonal
Preparation	This MERTK antibody is generated from rabbits immunized with a his tag recombinant protein of human MERTK.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

MERTK is a member of the MER/AXL/TYRO3 receptor kinase family and a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. Mutations in MERTK gene have been associated with disruption of the retinal pigment epithelium (RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa (RP).

Specificity

Other Names

MERTK; MER; Tyrosine-protein kinase Mer; Proto-oncogene c-Mer; Receptor tyrosine kinase MerTK

NCBI Accession #

NP_006334.2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

110249

Recommended Dilutions

WB: 1:1000; IF: 1:10-50; IHC: 1:50-10; FC: 1:10-50

	MER Antibody (Cat# 102-17664) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the MER antibody detected the MER protein (arrow).
	Fluorescent image of U251 cell stained with MERTK Antibody(Cat# 102-17664/SH090316F).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MERTK primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).MERTK immunoreactivity is localized to Vesicles significantly.
	Formalin-fixed and paraffin-embedded human lymphoma reacted with MERTK Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Flow cytometric analysis of jurkat cells using MERTK Antibody(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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