Anti-MGAT3 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	4248
Protein Name / Synonyms	MGAT3
Concentration (lot specific)	0.96 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

FLJ43371 antibody, GNT-III antibody, GNT3 antibody, MGC141943 antibody, MGC142278 antibody, MGAT3 antibody, N-acetylglucosaminyltransferase III antibody, "beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase antibody", GlcNAc-T III antibody, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III antibody, "mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase antibody"

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

61 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 260 and 525 of MGAT3 (Uniprot ID# Q09327)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed HeLa, using GnT-III(107-11008) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using MGAT3(107-11008) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: Hep G2

7.5% SDS PAGE
107-11008 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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