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Anti-MGMT Antibody
Catalog #: 102-20150
Mouse Anti-MGMT Antibody, 200 µl
Product Description
Specifications
| Size | 200 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Mouse |
| Accession Number | P16455 |
| Gene Id | 4255 |
| Gene Symbols | MGMT |
| Protein Name / Synonyms | Methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, MGMT, O-6-methylguanine-DNA-alkyltransferase, MGMT |
| Clonality | Monoclonal |
| Clone | 1527CT158.87.44 |
| Isotype | Kappa, IgG1 |
| Recommended Applications | FC, Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Other Information
Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Calculated Molecular Weight (Da)
21646
Antigen Source
Human
Images
FC (1:25)
Overlay histogram showing Jurkat cells stained with 102-20150 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (102-20150, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821)) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG (1?g/1x10
Expiration:
12 months from the date of shipment when stored properly.