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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 375056 |
| Protein Name / Synonyms | MIA3, N-term |
| Concentration (lot specific) | 0.3 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
ARNT antibody, D320 antibody, FLJ21469 antibody, FLJ39207 antibody, KIAA0268 antibody, TANGO antibody, TANGO1 antibody, UNQ6077 antibody, MIA3 antibody, melanoma inhibitory activity protein 3 antibody, C219-reactive peptide antibody, C219 reactive peptide antibody, transport and Golgi organization protein 1 antibody, "melanoma inhibitory activity family, member 3 antibody"
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
214 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 150 of MIA3 (Uniprot ID# Q5JRA6)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
MIA3 antibody [N1], N-term detects MIA3 protein at Cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in -20? 100% MeOH for 5 min.
Green: MIA3 protein stained by MIA3 antibody [N1], N-term (107-10292) diluted at 1:500.
Blue: Hoechst 33343 staining.
Sample: HepG2 cells were fixed in -20? 100% MeOH for 5 min.
Green: MIA3 protein stained by MIA3 antibody [N1], N-term (107-10292) diluted at 1:500.
Blue: Hoechst 33343 staining.
Immunohistochemical analysis of paraffin-embedded Testis, using MIA3(107-10292) antibody(10 µg/ml).
Sample (30 ug of whole cell lysate)
A: A549
B: Hep G2
5% SDS PAGE
107-10292 diluted at 1:1000
A: A549
B: Hep G2
5% SDS PAGE
107-10292 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.
