Rabbit Anti-Human Mib1/Mindbomb (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q86YT6 |
| Gene Id | 57534 |
| Gene Symbols | MIB1 |
| Protein Name / Synonyms | Mib1/Mindbomb (N-term) |
| Clonality | Polyclonal |
| Preparation | This Mib1/Mindbomb antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human Mib1/Mindbomb. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IF, IHC-P |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
MIB is an E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. This protein positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. MIB probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.
Specificity
Predicted Reactivity
Mouse, Zebrafish, Xenopus
Other Names
MIB1; DIP1; KIAA1323; ZZANK2; E3 ubiquitin-protein ligase MIB1; DAPK-interacting protein 1; Mind bomb homolog 1; Zinc finger ZZ type with ankyrin repeat domain protein 2
NCBI Accession #
NP_065825.1
Other Accession #
Q6GNY1; Q80SY4; Q804S5
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
110136
Recommended Dilutions
WB: 1:1000; IF: 1:20-100; IHC: 1:50-100
 | MIB Antibody (K28) (Cat# 102-15004) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the MIB antibody detected the MIB protein (arrow). |
 | Immunofluorescent staining of Hela cells incubated with MIB Antibody (N-term) (Cat# 102-15004) at a dilution of 1:20. Data courtesy of Dr. Vyacheslav Akimov, University of Southern Denmark. |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.