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Anti-MID1IP1 Antibody
Catalog #: 107-10652
MID1IP1 antibody, 0.68 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 58526 |
| Protein Name / Synonyms | MID1IP1 |
| Concentration (lot specific) | 0.68 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
FLJ10386 antibody, G12-like antibody, MIG12 antibody, STRAIT11499 antibody, THRSPL antibody, MID1IP1 antibody, MID1 interacting protein 1 (gastrulation specific G12-like) antibody, gastrulation-specific G12-like protein antibody, spot 14-R antibody, mid1-interacting G12-like protein antibody, MID1 interacting G12-like protein antibody, spot 14-related protein antibody, mid1-interacting protein 1 antibody, MID1 interacting protein 1 (gastrulation specific G12 homolog (zebrafish)) antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
20 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 183 of MID1IP1 (Uniprot ID# Q9NPA3)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using MID1IP1(107-10652) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using MID1IP1(107-10652) antibody at 1:100 dilution.
Sample(30 ug whole cell lysate)
A:293T
B:Hep G2
12% SDS PAGE
107-10652 diluted at 1:1000
A:293T
B:Hep G2
12% SDS PAGE
107-10652 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.