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Anti-MITF Antibody
Catalog #: 144-01255
MITF Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | O75030 |
| Gene Id | 4286 |
| Gene Symbols | MITF |
| Protein Name / Synonyms | MITF, CMM8, COMMAD, MI, WS2, WS2A, bHLHe32, melanogenesis associated transcription factor |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
40kDa/46kDa/52kDa/55kDa/56kDa/57kDa/58kDa
Observed MW
65kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human MITF (NP_937801.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using MITF antibody (144-01255) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Expiration:
12 months from the date of shipment when stored properly.