Anti-MLM (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q8N726
Gene Id	1029
Gene Symbols	CDKN2A
Protein Name / Synonyms	MLM (C-term)
Clonality	Polyclonal
Preparation	This MLM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-101 amino acids from the C-terminal region of human MLM.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, MDM1, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.

Specificity

Other Names

CDKN2A; CDKN2; MLM; Cyclin-dependent kinase inhibitor 2A, isoform 4; p14ARF; p19ARF

NCBI Accession #

NP_478102.2

Other Accession #

NP_478102.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

13903

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; FC: 1:10-50

	MLM Antibody (C-term) (Cat# 102-11778) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the MLM antibody detected the MLM protein (arrow).
	MLM Antibody (C-term) (Cat# 102-11778)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MLM Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	MLM Antibody (C-term) (Cat# 102-11778) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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