3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-MMP1 Antibody
Catalog #: 130-10016
Rabbit Anti-Human MMP1 Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P03956 |
| Gene Id | 4312 |
| Gene Symbols | MMP1 CLG |
| Clonality | Polyclonal |
| Immunogen | Immunogen was a partial length of recombinant protein of human Matrix Metalloproteinase 1. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation, and followed by Protein A/G affinity chromatography. |
| Recommended Applications | ELISA: Dilution = 1:160, 000 (at least detecting 6.25 ng/ml, Western Blotting: Dilution = 1:1, 000-5, 000, Immunodetection Analysis: Representative blot image below from a previous lot. The membrane blot was probed with antiMMP1 primary antibody (0.25 µg/ml). Recombinant proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular MMP1 from human and mouse cells (45 kDa)., |
| Antigen Catalog Number | RB-15-0006P |
| Reconstitution | Supplied as lyophilized purified antibody. The antibody is stable for at least one year from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored at -20°C to -70°C in a manual defrost freezer for years without losing detectable activity. Upon reconstitution, the antibody can be stored at 4°C for months. If 1 ml of water is used, the antibody concentration will be 100 µg/ml. Please avoid freeze-thaw cycles.. |
| Research Area | Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
MMP-1 is a member of the large family of matrix metalloproteinase (MMP) that degrades substrates, such as gelatin, nidogen, collagen type 3, collagen type 4, laminin-1, proteoglycans, elastin, etc. Most MMP proteins are activated after cleaved by extracellular proteinases. The matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological pathway: embryonic development, reproduction, tissue remodeling. MMP also involves in disease processes, such as arthritis and metastasis.
Specificity
The rabbit anti-matrix metalloproteinase 1 antibody specifically detected target derived from human recombinant protein as well as selected cell lysates. Cross reactivity with mouse and rat MMP1 has not yet been tested.
Storage
Store at -20°C, if not intended for use within a month, keep it at 4°C and minimize freezing and thawing when use.
Related Products
MMP-1 ELISA Kit (Cat# ELH-MMP1-001) MMP-1 ELISA Kit (for Lysates) (Cat# ELH-MMP1-001C) Matrix metalloproteinase 1 (interstitial collagenase) (Cat# RB-15-0006P-3)
References
- Tolboom, TC,et al. (2002)Invasive properties of fibroblast-like synoviocytes: correlation with growth characteristics and expression of MMP-1, MMP-3, and MMP-10. Annals of the Rheumatic Diseases 61(11): 975-980.
Expiration:
12 months from the date of shipment when stored properly.