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Anti-MMP1 Antibody, Biotinylated
Catalog #: 130-10016B-50
Rabbit anti-MMP1 Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P03956 |
| Gene Id | 4312 |
| Gene Symbols | MMP1 CLG |
| Clonality | Polyclonal |
| Immunogen | Immunogen was a partial length of recombinant protein of human Matrix Metalloproteinase 1. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation, and followed by Protein A/G affinity chromatography. |
| Isotype | IgG |
| Recommended Applications | ELISA: Dilution = 1:160, 000 (at least detecting 6.25 ng/ml, Western Blotting: Dilution = 1:1, 000-5, 000, Immunodetection Analysis: Representative blot image below from a previous lot. The membrane blot was probed with antiMMP1 primary antibody (0.25 µg/ml). Recombinant proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular MMP1 from human and mouse cells (45 kDa)., |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
MMP-1 is a member of the large family of matrix metalloproteinase (MMP) that degrades substrates, such as gelatin, nidogen, collagen type 3, collagen type 4, laminin-1, proteoglycans, elastin, etc. Most MMP proteins are activated after cleaved by extracellular proteinases. The matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological pathway: embryonic development, reproduction, tissue remodeling. MMP also involves in disease processes, such as arthritis and metastasis.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The rabbit anti-matrix metalloproteinase 1 antibody specifically detected target derived from human recombinant protein as well as selected cell lysates. Cross reactivity with mouse and rat MMP1 has not yet been tested.
Related Products
MMP-1 ELISA Kit (Cat# ELH-MMP1-001) MMP-1 ELISA Kit (for Lysates) (Cat# ELH-MMP1-001C) Matrix metalloproteinase 1 (interstitial collagenase) (Cat# RB-15-0006P-3)
References
- Tolboom, TC,et al. (2002)Invasive properties of fibroblast-like synoviocytes: correlation with growth characteristics and expression of MMP-1, MMP-3, and MMP-10. Annals of the Rheumatic Diseases 61(11): 975-980.
Expiration:
12 months from the date of shipment when stored properly.