Anti-MMP12 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P39900
Gene Id	4321
Gene Symbols	MMP12
Protein Name / Synonyms	MMP12 (C-term)
Clonality	Polyclonal
Preparation	This MMP12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 391-420 amino acids from the C-terminal region of human MMP12.
Concentration (lot specific)	1.560 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP12 may be involved in tissue injury and remodeling. This protein has significant elastolytic activity. MMP12 can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, wtih small hydrophobic residues (prefereably alanine) occupying P3. The protein is found in alveolar macrophages but not in peripheral blood monocytes. MMP12 can be induced by exposure to lypopolysaccharide, and is inhibited by dexamethasone.

Specificity

Other Names

MMP12; HME; Macrophage metalloelastase; Macrophage elastase; Matrix metalloproteinase-12

NCBI Accession #

NP_002417.2

Other Accession #

NP_002417

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

54002

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; IF: 1:10-50

	Western blot analysis of anti-hMMP12-R406 Pab (Cat# 102-16673) pre-incubated without(lane 1) and with(lane 2) blocking peptide in 293 cell line lysate. MMP12(arrow) was detected using the purified Pab;
	Western blot analysis of anti-hMMP12-R406 Pab (Cat# 102-16673) pre-incubated without(lane 1) and with(lane 2) blocking peptide in mouse kidney tissue lysates. MMP12(arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MMP12 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of MMP12 Antibody (C-term) (Cat# 102-16673) with 293 cell followed by Alexa Fluor^® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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