Anti-MMP14 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P50281
Gene Id	4323
Gene Symbols	MMP14
Protein Name / Synonyms	MMP14 (N-term)
Clonality	Polyclonal
Preparation	This MMP14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-174 amino acids from the N-terminal region of human MMP14.
Concentration (lot specific)	1.280 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Angiogenesis, Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP14 seems to specifically activate progelatinase A, and may thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. Expression is significant in stromal cells of colon, breast, and head and neck.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine, Pig

Other Names

MMP14; Matrix metalloproteinase-14; MMP-X1; Membrane-type matrix metalloproteinase 1; Membrane-type-1 matrix metalloproteinase

NCBI Accession #

NP_004986.1

Other Accession #

Q10739; Q9XT90; P53690; Q9GLE4; NP_004986

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

65894

Recommended Dilutions

WB: 1:1000; FC: 1:10-50; IHC: 1:50-100

	MMP14 Antibody (R160) (Cat# 102-16674) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the MMP14 antibody detected the MMP14 protein (arrow).
	Flow cytometric analysis of MCF-7 cells using MMP14 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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