Anti-MMP3 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P08254
Gene Id	4314
Gene Symbols	MMP3
Protein Name / Synonyms	MMP3 (Center)
Clonality	Polyclonal
Preparation	This MMP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 298-327 amino acids from the Central region of human MMP3.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Cardiovascular Disease, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP3 is an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation.

Specificity

Other Names

MMP3; STMY1; Stromelysin-1; Matrix metalloproteinase-3; Transin-1

NCBI Accession #

NP_002413.1

Other Accession #

NP_002413

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

53977

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; FC: 1:10-50

	Western blot analysis of MMP3 (arrow) using rabbit polyclonal MMP3 Antibody (Center) (Cat# 102-16679). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MMP3 gene (Lane 2) (Origene Technologies).
	Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MMP3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Flow cytometric analysis of HepG2 cells using MMP3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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