Anti-MMP9 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P14780
Gene Id	4318
Gene Symbols	MMP9
Protein Name / Synonyms	MMP9 (C-term)
Clonality	Polyclonal
Preparation	This MMP9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 644-673 amino acids from the C-terminal region of human MMP9.
Concentration (lot specific)	2.000 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Cardiovascular Disease, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.

Specificity

Other Names

MMP9; CLG4B; Matrix metalloproteinase-9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; 67 kDa matrix metalloproteinase-9; 82 kDa matrix metalloproteinase-9

NCBI Accession #

NP_004985.2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

78458

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	MMP9 Antibody (C-term) (Cat# 102-16681) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the MMP9 antibody detected the MMP9 protein (arrow).
	Western blot analysis of MMP9 (arrow) in mouse spleen lysates (35ug/lane). MMP9 (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	Formalin-fixed and paraffin-embedded human Spleen tissue reacted with MMP9 antibody (C-term) (Cat# 102-16681), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MMP9 antibody (C-term) (Cat# 102-16681), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.