Anti-Mouse APP (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P05067
Gene Id	351
Gene Symbols	APP
Protein Name / Synonyms	APP (N-term)
Clonality	Polyclonal
Preparation	This APP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-61 amino acids from the N-terminal region of human APP.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Neuroscience, Cardiovascular Disease, Cancer, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

APP is a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy).

Specificity

Predicted Reactivity

Monkey

Other Names

APP; A4; AD1; Amyloid beta A4 protein; ABPP; APPI; Alzheimer disease amyloid protein; Cerebral vascular amyloid peptide; PreA4; Protease nexin-II; N-APP; Soluble APP-alpha; Soluble APP-beta; C99; Beta-amyloid protein 42; Beta-APP42; Beta-amyloid protein 40; Beta-APP40; C83; P3(42); P3(40); C80; Gamma-secretase C-terminal fragment 59; Amyloid intracellular domain 59; Gamma-CTF(59); Gamma-secretase C-terminal fragment 57; Amyloid intracellular domain 57; Gamma-CTF(57); Gamma-secretase C-terminal fragment 50; Amyloid intracellular domain 50; Gamma-CTF(50); C31

NCBI Accession #

NP_000475.1;NP_001129488.1;NP_001129601.1;NP_001129602.1;NP_001129603.1;NP_001191230.1;NP_001191231.1;NP_001191232.1;NP_958816.1;NP_958817.1

Other Accession #

P53601

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

86943

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; FC: 1:10-50

	Western blot analysis of APP (arrow) using rabbit polyclonal APP Antibody (N-term) (RB05012). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the APP gene (Lane 2) (Origene Technologies).
	Formalin-fixed and paraffin-embedded human brain with APP Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Flow cytometric analysis of HepG2 cells using APP Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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