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Anti-Mouse CD11b Antibody, APC
Catalog #: 135-25001
Human / Mouse CD11b Antibody [clone: M1/70.15.11.5.HL], monoclonal rat IgG2b kappa. Validated for flow cytometry.
Product Description
Specifications
| Size | 25 µg, 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human, Mouse |
| Host Species | Rat |
| Protein Name / Synonyms | CD11b, integrin alpha-M (ITGAM), CR3A |
| Clonality | Monoclonal |
| Clone | M1/70.15.11.5.HL |
| Conjugation | APC |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG2b Kappa |
| Format | Liquid |
| Concentration (lot specific) | 0.5 mg/ml |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease, Wnt-beta-Catenin Signaling |
| Excitation Laser | Red Laser, 633 nm |
| Maximum Emission Wavelength | 676 nm |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Description
CD11b, also known as integrin alpha-M (ITGAM) and CR3A, is a 170 kDa glycoprotein primarily expressed on myeloid cells such as granulocytes, monocytes, macrophages, dendritic cells, NK cells and subsets of T/ B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form a heterodimer αMβ2, also called macrophage-1 antigen (Mac-1) or complement receptor 3 (CR3). Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Store at 2 °C ~ 8 °C, protected from prolonged light exposure. DO NOT FREEZE.