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Anti-Mouse CD11b Antibody, RayBright® Violet 450
Catalog #: 135-08001
RayBright® Violet 450 Mouse CD11b Antibody [clone: M1/70], monoclonal Rat IgG2b kappa. Validated for flow cytometry.
Product Description
Specifications
| Size | 25 µg, 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Mouse |
| Host Species | Rat |
| Protein Name / Synonyms | CD11b |
| Clonality | Monoclonal |
| Clone | M1/70 |
| Conjugation | RayBright® Violet 450 |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with a high quality fluorochrome, RayBright® Violet 450 |
| Isotype | IgG2b Kappa |
| Format | Liquid |
| Concentration (lot specific) | 0.5 mg/ml |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease |
| Excitation Laser | Violet Laser, 407 nm |
| Maximum Emission Wavelength | 454 nm, replace Pacific Blue™ |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Typical Data
Description
CD11b, also known as integrin alpha-M (ITGAM) and CR3A, is a 170 kDa glycoprotein primarily expressed on myeloid cells such as granulocytes, monocytes, macrophages, dendritic cells, NK cells and subsets of T/ B cells. CD11b non-covalently associates with CD18 (ÃŽ²2 integrin) to form a heterodimer ÃŽ±MÃŽ²2, also called macrophage-1 antigen (Mac-1) or complement receptor 3 (CR3). Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.
Product Details
Other Names
integrin alpha-M (ITGAM), CR3A
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ‚¬°¤ 0.5 µg per 106 cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage/Stability
Stored at 2°C ~ 8°C, protected from prolonged light exposure. DO NOT FREEZE.