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Anti-Mouse CD11c Antibody, APC
Catalog #: 135-25002
Mouse CD11c Antibody [clone: N418], monoclonal Hamster IgG kappa. Validated for flow cytometry.
Product Description
Specifications
| Size | 25 µg, 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Mouse |
| Host Species | Hamster |
| Protein Name / Synonyms | CD11c, αX integrin, CR4, and p150 |
| Clonality | Monoclonal |
| Clone | N418 |
| Conjugation | APC |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG Kappa |
| Format | Liquid |
| Concentration (lot specific) | 0.5 mg/ml |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease, Wnt-beta-Catenin Signaling |
| Excitation Laser | Red Laser, 633 nm |
| Maximum Emission Wavelength | 676 nm |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Description
CD11c, also known as αX integrin, CR4, and p150, is a 150 kDa glycoprotein. The mouse CD11c antigen is expressed on dendritic cells, macrophages and a subset of intestinal intraepithelial lymphocytes (IEL). It is reported to be weakly expressed on NK cells, B cells, and some activated T cells. CD11c is widely used as a pan-dendritic cell marker.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Store at 2 °C ~ 8 °C, protected from prolonged light exposure. DO NOT FREEZE.