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Anti-Mouse CD154 Antibody, RayBright® Red 647
Catalog #: 135-24062
Mouse CD154 Antibody [clone: MR1 [derivative of HB-11048]], monoclonal Hamster IgG. Validated for flow cytometry.
Product Description
Specifications
| Size | 25 µg, 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Mouse |
| Host Species | Hamster |
| Accession Number | P27548 |
| Gene Id | 21947 |
| Gene Symbols | Cd40lg, Cd40l, Tnfsf5 |
| Protein Name / Synonyms | CD40 ligand (CD40-L) (T-cell antigen Gp39) (TNF-related activation protein) (TRAP) (Tumor necrosis factor ligand superfamily member 5) (CD antigen CD154) [Cleaved into: CD40 ligand, membrane form; CD40 ligand, soluble form (sCD40L)] |
| Clonality | Monoclonal |
| Clone | MR1 [derivative of HB-11048] |
| Conjugation | RayBright® Red 647 |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG |
| Format | Liquid |
| Concentration (lot specific) | 0.5 mg/ml |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease, Inflammation |
| Excitation Laser | Red Laser, 633 nm |
| Maximum Emission Wavelength | 664 nm, replace Cy5™ and Alexa Fluor™ 647 (Cy5™ and Alexa Fluor™ are the trademark of Invitrogen and GE Health Care) |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Description
CD154, also known as CD40 Ligand, is an accessory molecule expressed on activated CD4+ lymphocytes. CD154 mediates T and B lymphocytes costiumlation. It also regulates the interations between lymphocytes and antigen-presenting cells.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Store at 2 °C ~ 8 °C, protected from prolonged light exposure. DO NOT FREEZE.