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Anti-Mouse CD19 Antibody, Biotinylated
Catalog #: 135-01006
Biotinylated Mouse CD19 Antibody [clone: 1D3], monoclonal Rat IgG2a kappa. Validated for flow cytometry.
Product Description
Specifications
| Size | 100 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Mouse |
| Host Species | Rat |
| Gene Symbols | CD19 |
| Clonality | Monoclonal |
| Clone | 1D3 |
| Conjugation | Biotin |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with biotin |
| Isotype | IgG2a Kappa |
| Format | Liquid |
| Concentration (lot specific) | 0.5 mg/ml |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Description
CD19, a member of the Ig superfamily, is a 95 kDa glycoprotein that is expressed on B cells from the earliest recognizable B-lineage cells during development to B-cell blasts but is downregulated in plasma cells. CD19 acts as a B cell co-receptor associated with CD21 and CD81, which form a molecular complex for B cell activation. CD19 is also expressed on follicular dendritic cells and peritoneal mast cells. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated, which leads to binding by Src-family kinases and recruitment of PI-3 kinase. CD19 and CD45R/B220 are both B cell markers.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Stored at 2 °C - 8 °C, protected from prolonged light exposure. DO NOT FREEZE.