Anti-Mouse CD19 Antibody, Purified

CD19, a member of the Ig superfamily, is a 95 kDa glycoprotein that is expressed on B cells from the earliest recognizable B-lineage cells during development to B-cell blasts but is downregulated in plasma cells. CD19 acts as a B cell co-receptor associated with CD21 and CD81, which form a molecular complex for B cell activation. CD19 is also expressed on follicular dendritic cells and peritoneal mast cells. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated, which leads to binding by Src-family kinases and recruitment of PI-3 kinase. CD19 and CD45R/B220 are both B cell markers.

Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.

Keep the lyophilized antibodies at -20 °C. Once reconstituted with PBS, store at 2 °C - 8 °C for less than 3 weeks or freeze at -20°C for long term storage. Please limit freeze and thaw cycles.