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Anti-Mouse CD25 Antibody, RayBright® Blue 488
Catalog #: 135-14010
RayBright® Blue 488 Mouse CD25 Antibody [clone: PC61], monoclonal Rat IgG1 lambda. Validated for flow cytometry.
Product Description
Specifications
| Size | 25 µg, 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Mouse |
| Host Species | Rat |
| Gene Symbols | CD25 |
| Protein Name / Synonyms | CD25 |
| Clonality | Monoclonal |
| Clone | PC61 |
| Conjugation | RayBright® Blue 488 |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with a high quality fluorochrome, RayBright® Blue 488 |
| Isotype | IgG1 Lambda |
| Format | Liquid |
| Concentration (lot specific) | 0.5 mg/ml |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease |
| Excitation Laser | Blue Laser, 488 nm |
| Maximum Emission Wavelength | 518 nm, replace FITC, Alexa Fluor™ 488, and BD BB515 (Alexa Fluor™ is the trademark of Invitrogen) |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Typical Data
Description
CD25, also named IL-2Rα, is an α chain of the interleukin 2 receptor (IL-2R). IL-2Rα associates with the IL-2Rβ chain (CD122) and the commonγ chain (CD132) to form the functional IL-2R complex. CD25 is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. Though CD25 has been used as a marker to identify CD4+FoxP3+ regulatory T cells in mice, it has been found that a large proportion of resting memory T cells constitutively express CD25 in humans.
Product Details
Other Names
IL-2Rα
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per 106 cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage/Stability
Stored at 2°C ~ 8°C, protected from prolonged light exposure. DO NOT FREEZE.