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Anti-Mouse CD28 Antibody, Purified
Catalog #: 135-00039
Mouse CD28 Antibody [clone: PV1], monoclonal Hamster IgG. Validated for flow cytometry.
Product Description
Specifications
| Size | 100 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Mouse |
| Host Species | Hamster |
| Protein Name / Synonyms | Tp44 |
| Clonality | Monoclonal |
| Clone | PV1 |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG |
| Format | Lyophilized |
| Concentration (lot specific) | Lyophilized |
| Buffers | PBS, pH 7.2-7.4; Carrier-free |
| Recommended Applications | Flow Cytometry |
| Reconstitution | Reconstitute in desired amount of PBS. |
| Research Area | Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
- Carrier free
- Endotoxin-free
Description
CD28 a 44 kD glycoprotein, is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells, acting as a co-stimulatory receptor of T cell activation. CD28 is expressed on resting T-cells, while the co-inhibitory receptor CTLA-4 is expressed as a on activated T-cells. Both molecules bind CD80 and CD86 on antigen presenting cells, but CTLA4 has higher affinity then CD28. Anti-CD28 along with anti-CD3 can also activate T cells in vitro.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Keep the lyophilized antibodies at -20 °C. Once reconstituted with PBS, store at 2 °C - 8 °C for less than 3 weeks or freeze at -20°C for long term storage. Please limit freeze and thaw cycles.