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Anti-Mouse CD326 Antibody, Purified
Catalog #: 135-00043
Mouse CD326 Antibody [clone: G8.8], monoclonal Rat IgG2a kappa. Validated for flow cytometry.
Product Description
Specifications
| Size | 100 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Mouse |
| Host Species | Rat |
| Protein Name / Synonyms | Ep-CAM |
| Clonality | Monoclonal |
| Clone | G8.8 |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG2a Kappa |
| Format | Lyophilized |
| Concentration (lot specific) | Lyophilized |
| Buffers | PBS, pH 7.2-7.4; Carrier-free |
| Recommended Applications | Flow Cytometry |
| Reconstitution | Reconstitute in desired amount of PBS. |
| Research Area | Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
- Carrier free
- Endotoxin-free
Description
CD326, also known as Ep-CAM, a 40kd type I transmembrane glycoprotein is a human epithelial cell adhesion molecule. CD326 is highly expressed in bone marrow, colon, lung, and most normal epithelial cells and on carcinomas of gastrointestinal origin. CD326 plays roles in several cellular processes, including signaling, migration, proliferation. In human being, CD326 antigen is an immunotherapeutic target for the treatment of human carcinomas. Anti-CD326 is widely used as marker for epithelial cells.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Keep the lyophilized antibodies at -20 °C. Once reconstituted with PBS, store at 2 °C - 8 °C for less than 3 weeks or freeze at -20°C for long term storage. Please limit freeze and thaw cycles.