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Anti-Mouse CD45 Antibody, APC
Catalog #: 135-25040
Mouse CD45 Antibody [clone: M1/9], monoclonal rat IgG2a kappa. Validated for flow cytometry.
Product Description
Specifications
| Size | 25 µg, 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Mouse |
| Host Species | Rat |
| Accession Number | P06800 |
| Gene Id | 19264 |
| Gene Symbols | Ptprc, Ly-5 |
| Protein Name / Synonyms | Receptor-type tyrosine-protein phosphatase C (EC 3.1.3.48) (Leukocyte common antigen) (L-CA) (Lymphocyte antigen 5) (Ly-5) (T200) (CD antigen CD45) |
| Clonality | Monoclonal |
| Clone | M1/9 |
| Conjugation | APC |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG2a Kappa |
| Format | Liquid |
| Concentration (lot specific) | 0.5 mg/ml |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease, Cancer, Wnt-beta-Catenin Signaling |
| Excitation Laser | Red Laser, 633 nm |
| Maximum Emission Wavelength | 676 nm |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Description
Mouse CD45, leukocyte common antigen (LCA), is a 180-240 kD type I transmembrane glycoprotein expressed on the surface of most hematopoietic cells except mature erythrocytes, platelets and plasma cells. CD45 is a member of the protein tyrosine phosphatase family and functions in a number of immunoregulatory processes, including B and T cell activation, growth, differentiation and oncogenic transformation. CD45 is a tyrosine phosphatase, that are involved in modulating the function of intracellular substrates such as the Src kinases Lck and Fyn. Alternative splicing of RA, RB and RC polypeptide sequences of CD45 gene results in 8 isoforms with molecular weight between 180 - 240 kDa, the expression of which differs according to cell type and functional status.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Store at 2 °C ~ 8 °C, protected from prolonged light exposure. DO NOT FREEZE.