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Anti-Mouse CD45R/B220 Antibody, PE-Cy7
Catalog #: 135-23019
Human / Mouse CD45R/B220 Antibody [clone: RA3-3A1/6.1], monoclonal rat IgG2b kappa. Validated for flow cytometry.
Product Description
Specifications
| Size | 25 µg, 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human, Mouse |
| Host Species | Rat |
| Protein Name / Synonyms | CD45R, B220 |
| Clonality | Monoclonal |
| Clone | RA3-3A1/6.1 |
| Conjugation | PE-Cy7 |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG2b Kappa |
| Format | Liquid |
| Concentration (lot specific) | 0.5 mg/ml |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease |
| Excitation Laser | Green Laser, 565 nm |
| Maximum Emission Wavelength | 778 nm |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Description
CD45R, also named B220, is one of the multiple isoforms of CD45, a protein tyrosine phosphatase receptor type C (PTPRC) that is an essential regulator of T- and B-cell antigen receptor signaling. CD45R/B220 is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, activated T cells, a subset of dendritic cells, other antigen-presenting cells and NK cells. In B cells, B220 plays a critical role in TCR and BCR signaling.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Store at 2 °C ~ 8 °C, protected from prolonged light exposure. DO NOT FREEZE.