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Anti-Mouse F4/80 Antibody, APC
Catalog #: 135-25027
Mouse F4/80 Antibody [clone: A3.1], monoclonal rat IgG2b kappa. Validated for flow cytometry.
Product Description
Specifications
| Size | 25 µg, 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Mouse |
| Host Species | Rat |
| Protein Name / Synonyms | F4/80, EMR1, Ly7 |
| Clonality | Monoclonal |
| Clone | A3.1 |
| Conjugation | APC |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG2b Kappa |
| Format | Liquid |
| Concentration (lot specific) | 0.5 mg/ml |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease, Wnt-beta-Catenin Signaling |
| Excitation Laser | Red Laser, 633 nm |
| Maximum Emission Wavelength | 676 nm |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Description
F4/80, also known as EMR1 or Ly7, is a 160 kDa glycoprotein and member of the adhesion GPCR family. F4/80 is a well-known and widely used marker of murine macrophage populations and is expressed by the majority of tissue macrophages including macrophages found in the lung, gut, peritoneal cavity, thymus, and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node, and Peyer's patch). Kuffer cells, Langerhans cells, bone marrow stromal cells, and subsets of dendritic cells also express F4/80. It has been reported that F4/80 is not necessary for the development of tissue macrophages but is required for the induction of efferent CD8+ regulatory T cells needed for peripheral tolerance.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Store at 2 °C ~ 8 °C, protected from prolonged light exposure. DO NOT FREEZE.