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Anti-Mouse FcgRII (CD32) Antibody, Biotinylated
Catalog #: 135-01005
Biotinylated Mouse Fc gamma RII/III Antibody [clone: 2.4G2], monoclonal Rat IgG2b kappa. Validated for flow cytometry.
Product Description
Specifications
| Size | 100 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Mouse |
| Host Species | Rat |
| Protein Name / Synonyms | FcγRII/III |
| Clonality | Monoclonal |
| Clone | 2.4G2 |
| Conjugation | Biotin |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with biotin |
| Isotype | IgG2b Kappa |
| Format | Liquid |
| Concentration (lot specific) | 0.5 mg/ml |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Description
CD16 is a low-affinity IgG Fc receptor III (FcR III), and CD32 is the high-affinity IgG Fc receptor FcR II. CD16/CD32 are expressed on natural killer cells, monocytes, macrophages, dendritic cells, Kupffer cells, granulocytes, mast cells, B lymphocytes, some bone marrow progenitor cells, immature thymocytes, and some activated mature T lymphocytes. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. It also mediates antibody-dependent cell-mediated cytotoxicity (ADCC). This antibody binds specifically to the extracellular domains of the mouse FcγIII and FcγII receptors. It also blocks non-antigen-specific binding of immunoglobulins to the FcγIII and FcγII, and possibly FcγI (CD64), receptors in vitro and in vivo.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Stored at 2 °C - 8 °C, protected from prolonged light exposure. DO NOT FREEZE.