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Anti-Mouse FcgRII (CD32) Antibody, RayBright® Red 647
Catalog #: 135-24005
RayBright® Red 647 Mouse Fc gamma RII/III Antibody [clone: 2.4G2], monoclonal Rat IgG2b kappa. Validated for flow cytometry.
Product Description
Specifications
| Size | 25 µg, 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Mouse |
| Host Species | Rat |
| Protein Name / Synonyms | CD16/CD32 |
| Clonality | Monoclonal |
| Clone | 2.4G2 |
| Conjugation | RayBright® Red 647 |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with a high quality fluorochrome, RayBright® Red 647 |
| Isotype | IgG2b Kappa |
| Format | Liquid |
| Concentration (lot specific) | 0.5 mg/ml |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease |
| Excitation Laser | Red Laser, 633 nm |
| Maximum Emission Wavelength | 664 nm, replace Cy5™ and Alexa Fluor™ 647 (Cy5™ and Alexa Fluor™ are the trademark of Invitrogen and GE Health Care) |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Typical Data
Description
CD16 is a low-affinity IgG Fc receptor III (FcR III), and CD32 is the high-affinity IgG Fc receptor FcR II. CD16/CD32 are expressed on natural killer cells, monocytes, macrophages, dendritic cells, Kupffer cells, granulocytes, mast cells, B lymphocytes, some bone marrow progenitor cells, immature thymocytes, and some activated mature T lymphocytes. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. It also mediates antibody-dependent cell-mediated cytotoxicity (ADCC). This antibody binds specifically to the extracellular domains of the mouse FcγIII and FcγII receptors. It also blocks non-antigen-specific binding of immunoglobulins to the FcγIII and FcγII, and possibly FcγI (CD64), receptors in vitro and in vivo.
Product Details
Other Names
FcγRII/III
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per 106 cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage/Stability
Stored at 2°C ~ 8°C, protected from prolonged light exposure. DO NOT FREEZE.