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Anti-Mouse IL-12 Antibody, Biotinylated
Catalog #: RB-IP-01-114B-50
Rat anti-Mouse IL-12 Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Mouse |
| Host Species | Rat |
| Immunogen | Immunogen was recombinant protein derived from mouse IL-12 p75. This antibody was produced from a hybridoma resulting from the fusion of a myeloma with B cells obtained from a rat immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G affinity chromatography. |
| Isotype | IgG2b |
| Recommended Applications | ELISA (recommended work dilution= At least 1:5000), Western Blotting (recommended work dilution= 1-2 µg/ml) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | Cardiovascular Disease, Inflammation |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
IL-12 is considered a critical proinflammatory cytokine for autoimmune diseases such as multiple sclerosis and its animal model, experimental autoimmune encephalomyelitis (EAE). IL-12 is a heterodimer composed of a p35 subunit and a common p40 subunit shared by other cytokines. Interleukin (IL)-12p75 is a heterodimeric cytokine composed of the product of two different genes that specify p35 and p40 subunits. Both IL-12 p40(-/-) and p35(-/-) mice fail to produce IL-12 p75 heterodimer. Studies show that a molecule other than IL-12 p75, which uses the p40 subunit, fulfills the functions previously attributed to IL-12 with regard to the development and pathogenesis of this autoimmune disease.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
This antibody was selected for its ability to detect mouse IL-12 in ELISA and Array. In ELISA, this antibody shows reactivity with IL-12 p40 and p75 and no cross-reactivity with murine IL-10 and other tested proteins.
References
- Peng, X., Mosser, D.M., Adler, M.W., Rogers, T.J., Meissler, J.J., Eisenstein, T.K. Morphine enhances interleukin-12 and the production of other pro-inflammatory cytokines in mouse peritoneal macrophages. J. Leukoc. Biol. (2000)
- Cooper, A.M., Kipnis, A., Turner, J., Magram, J., Ferrante, J., Orme, Mice lacking bioactive IL-12 can generate protective, antigen-specific cellular responses to mycobacterial infection only if the IL-12 p40 subunit is present. I.M. J. Immunol. (2002)
Expiration:
12 months from the date of shipment when stored properly.