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Anti-Mouse IL-12 Antibody
Catalog #: RB-IP-01-114
Rat Anti-Mouse IL-12 Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Mouse |
| Immunogen | Immunogen was recombinant protein derived from mouse IL-12 p75. This antibody was produced from a hybridoma resulting from the fusion of a myeloma with B cells obtained from a rat immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= At least 1:5000), Western Blotting (recommended work dilution= 1-2 µg/ml) |
| Reconstitution | Supplied as lyophilized purified antibody. It can be reconstituted with sterile water before use. Please avoid freeze-thaw cycles. |
| Research Area | Cardiovascular Disease, Inflammation |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
IL-12 is considered a critical proinflammatory cytokine for autoimmune diseases such as multiple sclerosis and its animal model, experimental autoimmune encephalomyelitis (EAE). IL-12 is a heterodimer composed of a p35 subunit and a common p40 subunit shared by other cytokines. Interleukin (IL)-12p75 is a heterodimeric cytokine composed of the product of two different genes that specify p35 and p40 subunits. Both IL-12 p40(-/-) and p35(-/-) mice fail to produce IL-12 p75 heterodimer. Studies show that a molecule other than IL-12 p75, which uses the p40 subunit, fulfills the functions previously attributed to IL-12 with regard to the development and pathogenesis of this autoimmune disease.
Specificity
This antibody was selected for its ability to detect mouse IL-12 in ELISA and Array. In ELISA, this antibody shows reactivity with IL-12 p40 and p75 and no cross-reactivity with murine IL-10 and other tested proteins.
Storage
Lyophilized: store at 4 C. After reconstitution, if not used within a month, aliquot and store at 20 C. Stability: Two years lyophilized, one month in solution at 4°C.
References
- Peng, X., Mosser, D.M., Adler, M.W., Rogers, T.J., Meissler, J.J., Eisenstein, T.K. Morphine enhances interleukin-12 and the production of other pro-inflammatory cytokines in mouse peritoneal macrophages. J. Leukoc. Biol. (2000)
- Cooper, A.M., Kipnis, A., Turner, J., Magram, J., Ferrante, J., Orme, Mice lacking bioactive IL-12 can generate protective, antigen-specific cellular responses to mycobacterial infection only if the IL-12 p40 subunit is present. I.M. J. Immunol. (2002)
Expiration:
12 months from the date of shipment when stored properly.