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Anti-Mouse IL-4 Antibody, Biotinylated
Catalog #: RB-IP-01-107B-50
Rat anti-Mouse IL-4 Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Mouse |
| Host Species | Rat |
| Accession Number | NP_067258 |
| Immunogen | Immunogen was whole expressing mouse IL-4. This antibody was obtained from a mice immunized with the immunogen, then fusion, selection and identification. This antibody was produced from a hybridoma resulting from the fusion of a myeloma with B cells deriveded from the immunized rat. The IgG fraction of cell culture supernatant was purified by Protein G affinity chromatography. |
| Isotype | IgG1 |
| Recommended Applications | ELISA (recommended work dilution= 1: 1, 000-2, 000), Western Blotting (recommended work dilution= 2-5 µg/ml) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | Cardiovascular Disease, Inflammation |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
IL-4 is a T cell product originally described as B cell growth factor by virtue of its ability to co-stimulate B lymphocyte proliferation (1). It has broad effects on B cells, including the promotion of Ig class switching to IgE and IgGl isotypes in activated B cells. In addition to regulating B lymphocyte growth and differentiation, IL-4 modulates the survival, proliferation, and differentiation of T lymphocytes, as well as a wide range of hematopoietic cell lineages, thereby contributing to allergic and other immune processes.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The antibody specifically detected protein derived from rat. It also recognizes tested samples containing IL-4 in both ELISA and WB. This antibody shows no cross-reactivity with human IL-4 and other unrelated proteins.
References
- Lebman, D. A., A. Lebman, and R. L. Coffman. 1988. Interleukin 4 causes isotype switching to IgE in T cell-stimulated clonal B cell cultures.J. Exp. Med. 168, 853 .
- Kepron, M. R., Y.W. Chen, J. W. Uhr, and E. S. Vitetta. 1989. IL-4 induces the specific rearrangement of yl genes on the expressed and unexpressed chromosomes of lipopolysaccharide-activated normal murine cells. f. Immunol. 143:334.
Expiration:
12 months from the date of shipment when stored properly.