Anti-Mouse MIP-2 / CXCL2 Antibody

Product Description

Specifications

Size	0.1 mg
Estimated Lead Time	1-2 weeks
Species	Mouse
Specificity	This product is specific for mouse Macrophage Inflammatory Protein-2 (MIP-2), also known as CXCL2. MIP-2 is expressed by macrophages and epidermal langerhans cells and is chemotactic for neutrophils.
Host Species	Rabbit
Protein Name / Synonyms	MIP-2 / CXCL2
Preparation	Antisera to mouse MIP-2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Concentration (lot specific)	IgG concentration 0.1 mg/ml after reconstitution.
Recommended Applications	Immunohistology - Frozen, ELISA (dilution: 0.1ug/ml, 0.2ug/ml), Functional Assays, Immunohistology - Paraffin, Western Blotting
Reconstitution	Reconstitute with 1.0 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide
Research Area	Cardiovascular Disease, Inflammation, Chemokine Biology
Shipping Type	Ambient temperature
Storage	-20°C

Description

This product may be used under either reducing or non-reducing conditions.

Immunohistology:

*This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

Sodium citrate buffer pH 6.0 is recommended for this purpose.

Storage/Stability

Prior to reconstitution store at +4 °C. Following reconstitution store at -20 °C.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.

Format

Supplied as:

Purified IgG - lyophilized

Formulation:

Phosphate buffered saline

Reconstitution:

Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide

Format Type:

Purified

Preparation

Antisera to mouse MIP-2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Immunogen:

Recombinant mouse MIP-2

Preservative:

None present

Expiration:

12 months from the date of shipment when stored properly.

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