Anti-Mouse NURR1 (NR4A2) (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P43354
Gene Id	4929
Gene Symbols	NR4A2
Protein Name / Synonyms	NURR1 (NR4A2) (N-term)
Clonality	Polyclonal
Preparation	This NURR1 (NR4A2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human NURR1 (NR4A2).
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	IHC-P, IF
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Parkinson's disease (PD) is a multifactorial disease that appears to arise from the effects of both genetic and environmental influences. The known genetic factors include multiple genes that have been identified in related parkinsonian syndromes, as well as alpha-synuclein. Genes associated with either PD or Parkinson-related disorders include parkin, DJ-1, ubiquitin C-terminal hydrolase isozyme L1 (UCH-L1), nuclear receptor-related factor 1 (NURR1), and alpha-synuclein. Nurr1 is a transcription factor that is expressed in the embryonic ventral midbrain and is critical for the development of dopamine (DA) neurons. It belongs to the conserved family of nuclear receptors but lacks an identified ligand and is therefore referred to as an orphan receptor. RXR ligands can promote the survival of DA neurons via a process that depends on Nurr1-RXR heterodimers. In developing DA cells, Nurr1 is required for the expression of several genes important for DA synthesis and function. Nurr1 is also important for the maintenance of adult DA neurons.

Specificity

Predicted Reactivity

Rat, Bovine

Other Names

NR4A2; NOT; NURR1; TINUR; Nuclear receptor subfamily 4 group A member 2; Immediate-early response protein NOT; Orphan nuclear receptor NURR1; Transcriptionally-inducible nuclear receptor

NCBI Accession #

NP_006177.1

Other Accession #

Q07917; Q06219; Q08E53

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

66591

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; IF: 1:10-50

	Western blot analysis of anti-NURR1(NR4A2) Pab (Cat# 102-16780) in mouse brain tissue lysate. NURR1(NR4A2) (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human brain tissue reacted with NURR1 (NR4A2) antibody (N-term)(Cat# 102-16780), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of NURR1 (NR4A2) Antibody (N-term) (Cat# 102-16780) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
	NR4A2 Antibody (N-term) (Cat# 102-16780) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NR4A2 antibody detected the NR4A2 protein (arrow).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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